TY  -  JOUR
AU  -  Serna Ortega, Paula Andrea
AU  -  Vailati, Francesca
AU  -  Milesi, Milena
AU  -  Manisco, Alberto
AU  -  Arosio, Marco
AU  -  Raglio, Annibale
T1  -  Resistenza di Klebsiella pneumoniae ai carbapenemi:<BR>test fenotipici versus metodi molecolari (gene blaKPC)
PY  -  2012
Y1  -  2012-07-01
DO  -  10.1716/1230.13632
JO  -  GIMPIOS
JA  -  Gimpios
VL  -  2
IS  -  3
SP  -  103
EP  -  107
PB  -  Il Pensiero Scientifico Editore
SN  -  1122-407X
Y2  -  2026/07/07
UR  -  http://dx.doi.org/10.1716/1230.13632
N2  -  Introduction. The wide distribution and the global spread of carbapenem non susceptible Enterobacteriaceae (CNSE) has become a serious problem in the community and in hospital, also in our local reality these strains are increasing. Among these, the KPC (Klesiella Pneumoniae Carbapenemase) strains producer are the big challenge for their detection. Today several methods allow the identification of KPC-producing strains using different techniques, traditional and molecular. The purpose of this study is to evaluate the performance of four molecular methods for the blaKPC gene detection in strains of Klebsiella pneumoniae CNS, in comparison with the phenotypic test in use in our laboratory. Methods. We evaluated 27 strains, 25 isolated in our laboratory and two ATCC reference strains, with different tests: Phenotypic tests: Modified Hodge Test (MHT), Double Disk Synergy Test (DDST), Combination Disk Test (CDT). Genotyping tests: “Home made” PCR,4 Hy-KPC Detection Real time Kit (Hy-labs®), Hy-KPC Detection PCR Kit (Hy-labs®), Easy Q KPC (BioMèrieux). Results and discussion. The results showed 100% concordance between the different phenotypic test and 100% agreement between phenotypic and genotypic methods, all with 100% of sensitivity and specificity. The phenotypic tests requires at least 24 hours for reporting, because they need for an overnight incubation, otherwise, molecular methods provide results in about 2 hours, as in the case of Hy-Lab Real Time PCR and of Easy Q KPC kits.
ER  -