TY  -  JOUR
AU  -  Collini, Lucia
AU  -  Bassetti, Danila
AU  -  Salvetti, F.
AU  -  Peruzzi, T.
AU  -  Franch, S.
AU  -  Bezzi, C.
AU  -  Palazzi, L.
AU  -  Predazzer, Rosanna
AU  -  Gaino, Marina
AU  -  D’Arcangelo, S.
AU  -  Lanzafame, Paolo
T1  -  Comparazione tra la diagnosi molecolare di infezione da Clostridium difficile con RT-PCR in microfluidica e un saggio immunoenzimatico<BR>per la ricerca della glutammato-deidrogenasi
PY  -  2013
Y1  -  2013-04-01
DO  -  10.1716/1345.14968
JO  -  GIMPIOS
JA  -  Gimpios
VL  -  3
IS  -  2
SP  -  80
EP  -  84
PB  -  Il Pensiero Scientifico Editore
SN  -  1122-407X
Y2  -  2026/07/06
UR  -  http://dx.doi.org/10.1716/1345.14968
N2  -  C. difficile is a major cause of health-care associated infections and is the principle cause of diarrhoea in hospitalised patients, particularly in those with a recent history of antibiotic use. C. difficile can cause a range of clinical symptoms from mild to severe diarrhoea and more seriously, pseudomembranous colitis, toxic megacolon, bowel perforation, sepsis and death. The incidence of C. difficile infection has been increasing in individuals considered at-risk; individuals in health care facilities, those with a recent history of hospitalisation, prior use of antibiotics or chemotherapeutics, the elderly and immunocompromised. In  this study were tested 93 samples of diarrheal stools with three different methods: a method of immunoassay (EIA) for the membrane protein-glutamate dehydrogenase (GDH), a method of molecular biology Real-Time and a cytotoxicity assay. 82 samples (88.2%) gave concordant results by both methods, 29 positive and 53 negative (GDH and PCR). Of the remaining 11 (11.8%), 4 tested positive for GDH and negative molecular method for GDH and 7 negative and positive RT-PCR. Samples with discordant results between the two methods have been subjected to culture on CCFA medium and neutralization tests of the cytotoxic cell culture (CCNA). The molecular method proved to be the quickest method, simple and accurate diagnostic protocol and the single step is the most effective in the diagnosis of infection with C. difficile even if significantly more expensive than other protocols.
ER  -